triton in pbs pbst (Thermo Fisher)

Thermo Fisher is a verified supplier

Thermo Fisher manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Thermo Fisher triton in pbs pbst
Triton In Pbs Pbst, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbst+buffer/Phosphate-buffered+saline+(PBS%2C+10X)%2C+with+Triton%26mid%3Br+X-100/pm42349735-148-20-17
Average 94 stars, based on 1 article reviews

triton in pbs pbst - by Bioz Stars, 2026-10

94/100 stars

Images

Incubation:

Article Title: Endothelial Zmiz1 modulates developmental angiogenesis in the retina
Article Snippet: .. Next, both yolk sacs and embryos were incubated in PBST buffer (PBS with 0.5% Triton X-100) overnight at 4 °C and then transferred into CAS-Block (Life technologies, 008120) for 4 h at room temperature. .. Both yolk sac tissues and whole embryos were incubated in primary antibody to rat anti-PECAM-1(BD Pharmingen, 553370) diluted in CAS-Block overnight at 4 °C.

Article Title: PD-1 antigen-binding protein and use thereof
Article Snippet: .. The plate was incubated at 37° C. for 1 h. After the plate was washed 3 times with PBST buffer (pH 7.4, containing 0.05% Tween-20), a 4000-fold diluted goat anti-human HRP secondary antibody (Invitrogen, #A18805) was added. .. The plate was incubated at 37° C. for 1 h. After the plate was washed, TMB (Biopanda, # TMB-S-003) was added at 100 μL/well.

Article Title: Cidea Targeting Protects Cochlear Hair Cells and Hearing Function From Drug- and Noise-Induced Damage.
Article Snippet: Primary antibodies included anti-Myo7a (Proteus Bioscience, #25-6790), anti-Sox2 (R&D Systems, #AF2018), and anti-Cidea (provided by Prof. Peng Li, Tsinghua University; Proteintech, #13170-1-AP). .. After three washes with PBST buffer (PBS containing 0.1% Triton X-100), the samples were incubated for 1 h at room temperature with either fluorescent-conjugated secondary antibodies (Invitrogen) or phalloidin (Invitrogen, #A22281) suspended in PBT2 (1% BSA and 0.1% Triton X-100 prepared in PBS at pH 7.4). .. Following additional washes with PBST, the cochleae were coverslipped with a commercial antifade mounting agent (DAKO, #S3023).

Article Title: Repression of PRMT activities sensitize human homologous recombination-proficient ovarian and breast cancer cells to PARP inhibitor treatment
Article Snippet: After four washes with PBST, coverslips were mounted onto glass slides using Vectashield mounting medium containing DAPI (Vector Laboratories) and visualized using an Axiovert 200 M inverted microscope (Zeiss). .. Cells were subsequently permeabilized with 0.5% Triton solution for 5 min at 4 °C and then incubated with PicoGreen (1:1000 dilution) (Cat No: P11496 , ThermoFisher Scientific) in PBST buffer (PBS plus 0.1% Tween-20, 0.02% NaN3) overnight at 4 °C. .. Cells were subsequently permeabilized with 0.5% Triton solution for 5 min at 4 °C and then incubated with PicoGreen (1:1000 dilution) (Cat No: P11496 , ThermoFisher Scientific) in PBST buffer (PBS plus 0.1% Tween-20, 0.02% NaN3) overnight at 4 °C.

Article Title: Statins regulate kinase signaling by causing changes in phosphorylation, rather than through changes in gene expression or direct inhibition: evidence in colorectal cancer
Article Snippet: .. After three washes with PBST buffer (PBS +0.1% Tween-20), membranes were incubated for 1 h at room temperature with a goat anti-mouse secondary antibody (Invitrogen, 31430), followed by additional PBST washes. ..

Concentration Assay:

Article Title: Conventional and non-conventional antigen-binding sites promote the development and function of chronic lymphocytic leukemia stereotyped subset #4 clones
Article Snippet: Non-specific binding was reduced by incubation with blocking buffer (100 mM Sodium Phosphate, pH 7.4, 200 mM NaCl, 0.08% Triton X100, 25% glycerol, 20 mM reduced glutathione, 1.0 mM DTT, 1% Hammarsten Grade casein) with gentle agitation in QuadriPERM 4-well trays (Greiner) for 1 hour at 4°C. .. Various antibodies were diluted in PBST buffer (1X PBS, 0.1% Tween 20, 1% Hammersten Grade casein) to a concentration of 10 and 50 μg/ml and added to arrays under a LifterslipTM (Erie Scientific). ..

Saline:

Article Title: Bromhexine inhibits SARS-CoV-2 Omicron and variant pseudovirus infection via ACE2-targeted mechanisms
Article Snippet: .. Proteins were then transferred to nitrocellulose membranes (Thermo Fisher Scientific) and blocked with PBST buffer (1x phosphate-buffered saline and 0.1% Tween-20) containing 5% non-fat milk overnight at 4 °C. .. After blocking, the membranes were incubated with primary antibodies against ACE2 (sc-390851, Santa Cruz Biotechnology), and Actin (sc-1615, Santa Cruz Biotechnology) at room temperature for 4 h. Subsequently, membranes were incubated with HRP-conjugated secondary antibodies at room temperature for 2 h, and the antibody-antigen complexes were detected using SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific).

Review

triton in pbs pbst (Thermo Fisher)

Structured Review

Images

Related Articles

Incubation:

Concentration Assay:

Saline:

Similar Products

Image Search Results